Translational Detection of Nonproteinogenic Amino Acids Using an Engineered Complementary Cell-Free Protein Synthesis Assay
نویسندگان
چکیده
منابع مشابه
In Vitro Mutasynthesis of Lantibiotic Analogues Containing Nonproteinogenic Amino Acids
Lantibiotics are ribosomally synthesized and post-translationally modified peptide antibiotics containing the characteristic thioether cross-links lanthionine and methyllanthionine. To date, no analogues of lantibiotics that contain nonproteinogenic amino acids have been reported. In this study, in vitro-reconstituted lacticin 481 synthetase was used in conjunction with synthetic peptide substr...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2020
ISSN: 0003-2700,1520-6882
DOI: 10.1021/acs.analchem.0c01978